Mice exposed in situ to urban air pollution exhibit pulmonary alterations in gene expression in the lipid droplet synthesis pathways
Identifieur interne : 000F77 ( Main/Exploration ); précédent : 000F76; suivant : 000F78Mice exposed in situ to urban air pollution exhibit pulmonary alterations in gene expression in the lipid droplet synthesis pathways
Auteurs : Andrea Rowan-Carroll [Canada] ; Sabina Halappanavar [Canada] ; Andrew Williams [Canada] ; Christophers M. Somers [Canada] ; Carole L. Yauk [Canada]Source :
- Environmental and Molecular Mutagenesis [ 0893-6692 ] ; 2013-05.
Abstract
It is clear that particulate air pollution poses a serious risk to human health; however, the underlying mechanisms are not completely understood. We investigated pulmonary transcriptional responses in mice following in‐situ exposure to ambient air in a heavily industrialized urban environment. Mature C57BL/CBA male mice were caged in sheds near two working steel mills and a major highway in Hamilton, Ontario, Canada in the spring/summer of 2004. Control mice were housed in the same environment, but received only high‐efficiency particle filtered air (HEPA). Whole lung tissues were collected from mice exposed for 3, 10, or for 10 weeks followed by 6 weeks recovery in the laboratory (16 weeks). DNA microarrays were used to profile changes in pulmonary gene expression. Transcriptional profiling revealed changes in the expression of genes implicated in the lipid droplet synthesis (Plin I, Dgat2, Lpl, S3‐12, and Agpat2), and antioxidant defense (Ucp1) pathways in mice breathing unfiltered air. We postulate that exposure to urban air, containing an abundance of particulate matter adsorbed with polycyclic aromatic hydrocarbons, triggers lipid droplet (holding depots for lipids and malformed/excess proteins tagged for degradation) synthesis in the lungs, which may act to sequester particulates. Increased lipid droplet synthesis could lead to endogenous/stressor‐induced production of reactive oxygen species and activation of antioxidant mechanisms. Further investigation into the stimulation of lipid droplet synthesis in the lung in response to air pollution and the resulting health implications is warranted. Environ. Mol. Mutagen. 54:240–249, 2013. © 2013 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/em.21768
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Mutagen.</title><idno type="ISSN">0893-6692</idno><idno type="eISSN">1098-2280</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><date type="published" when="2013-05">2013-05</date><biblScope unit="volume">54</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="240">240</biblScope><biblScope unit="page" to="249">249</biblScope></imprint><idno type="ISSN">0893-6692</idno></series><idno type="istex">BA9E1F5DA7E1674EAB4D152DAFEFF07D6C67C457</idno><idno type="DOI">10.1002/em.21768</idno><idno type="ArticleID">EM21768</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0893-6692</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">It is clear that particulate air pollution poses a serious risk to human health; however, the underlying mechanisms are not completely understood. We investigated pulmonary transcriptional responses in mice following in‐situ exposure to ambient air in a heavily industrialized urban environment. Mature C57BL/CBA male mice were caged in sheds near two working steel mills and a major highway in Hamilton, Ontario, Canada in the spring/summer of 2004. Control mice were housed in the same environment, but received only high‐efficiency particle filtered air (HEPA). Whole lung tissues were collected from mice exposed for 3, 10, or for 10 weeks followed by 6 weeks recovery in the laboratory (16 weeks). DNA microarrays were used to profile changes in pulmonary gene expression. Transcriptional profiling revealed changes in the expression of genes implicated in the lipid droplet synthesis (Plin I, Dgat2, Lpl, S3‐12, and Agpat2), and antioxidant defense (Ucp1) pathways in mice breathing unfiltered air. We postulate that exposure to urban air, containing an abundance of particulate matter adsorbed with polycyclic aromatic hydrocarbons, triggers lipid droplet (holding depots for lipids and malformed/excess proteins tagged for degradation) synthesis in the lungs, which may act to sequester particulates. Increased lipid droplet synthesis could lead to endogenous/stressor‐induced production of reactive oxygen species and activation of antioxidant mechanisms. Further investigation into the stimulation of lipid droplet synthesis in the lung in response to air pollution and the resulting health implications is warranted. Environ. Mol. Mutagen. 54:240–249, 2013. © 2013 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Saskatchewan</li></settlement><orgName><li>Université de Regina</li></orgName></list><tree><country name="Canada"><noRegion><name sortKey="Rowan Arroll, Andrea" sort="Rowan Arroll, Andrea" uniqKey="Rowan Arroll A" first="Andrea" last="Rowan-Carroll">Andrea Rowan-Carroll</name></noRegion><name sortKey="Halappanavar, Sabina" sort="Halappanavar, Sabina" uniqKey="Halappanavar S" first="Sabina" last="Halappanavar">Sabina Halappanavar</name><name sortKey="Halappanavar, Sabina" sort="Halappanavar, Sabina" uniqKey="Halappanavar S" first="Sabina" last="Halappanavar">Sabina Halappanavar</name><name sortKey="Somers, Christophers M" sort="Somers, Christophers M" uniqKey="Somers C" first="Christophers M." last="Somers">Christophers M. Somers</name><name sortKey="Williams, Andrew" sort="Williams, Andrew" uniqKey="Williams A" first="Andrew" last="Williams">Andrew Williams</name><name sortKey="Yauk, Carole L" sort="Yauk, Carole L" uniqKey="Yauk C" first="Carole L." last="Yauk">Carole L. Yauk</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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